Introduction to plant tissue culture

This is typically facilitated via use of a liquid, semi-solid, or solid growth mediumsuch as broth or agar. Tissue culture commonly refers to the culture of animal cells and tissues, while the more specific term plant tissue culture is being named for the plants. Historical usage In Wilhelm Roux removed a section of the medullary plate of an embryo nic chicken and maintained it in a warm saline solution for several days, establishing the basic principle of tissue culture. In the zoologist Ross Granville Harrison demonstrated the growth of frog nerve cell processes in a medium of clotted lymph.

Introduction to plant tissue culture

Introduction to plant tissue culture

Cashing in on Cloning Whoever said that variety is the spice of life must have been referring to the horticulture industry.

Finding and commercialising new and exciting plant varieties that will surprise and delight customers is essential in this business. That is where AgriForest Bio-Technologies can help nurseries, growers, and garden centres stay one step ahead of the game. AgriForest specialises in bringing unique or difficult to propagate varieties into production on a commercial scale over a brief time span.

What is Tissue Culture? How does AgriForest go about turning one plant with the desired characteristics into tens of thousands of identical plants in as fast as one year? Through its patented tissue culture protocols that it has developed over its twenty years in business.


Tissue culture is a process that involves exposing plant tissue to a specific regimen of nutrients, hormones, and light under sterile, in vitro conditions to produce many new plants, each a clone of the original mother plant, over a very short period of time.

There are three main steps to the tissue culture process. Following these three stages, the plants are then moved from the laboratory to the greenhouses for acclimatisation and further development.

Introduction to plant tissue culture

It concerns the establishment of plant tissue in vitro by sterilizing the material and initiating it into culture. At this stage, the in vitro plant material is re-divided and placed in a medium with plant growth regulators that induce the proliferation of multiple shoots.

This process is repeated many times until the number of plants desired is reached. It involves the introduction of hormones to induce rooting and the formation of complete plantlets. Plant tissue culture technology has proven itself to be an effective and viable option for growers to seriously consider in a variety of different situations.

When large-scale propagation of new or superior plant varieties is required for early introduction to market Following a decision to release a new variety into the market, the key to success for growers is rapid scale up and production on a commercial level.

Tissue culture is often the fastest and most economical means to achieve this goal.

Introduction to plant tissue culture | Adedotun Afolayan (PhD.) -

We suggest that growers continually compare the cost of conventional propagation methods to micropropagation, especially when conducted by an established tissue culture company with a proven track record of mass propagation such as AgriForest.

Give us a call or email us for detailed pricing information. We are certain that you will be pleased with our competitive rates! When mass multiplication is needed for varieties which are difficult to regenerate by conventional methods of propagation It is often the case that new or highly valued plant varieties are also the most difficult to propagate using traditional means.

Here again, tissue culture technology can be very helpful to growers. When disease-free plant propagation is important One of the inherent requirements of the tissue culture process is that it be conducted in sterile, aseptic conditions. This results in plants that are generally free of bacterial and fungal diseases.

This aspect of tissue culture is especially helpful for growers that are propagating plant varieties that have major systemic disease problems. Furthermore, tissue culture leads to the regeneration of whole plants, with their own full root systems and vigorous top growth like young seedlings.

Leave this field empty if you're human:Plant tissue culture - essential to plant biotechnology Animal Cell Culture: viruses as biological control agents and used in livestock breeding Test drugs and viruses. Plant Cell and Tissue Culture Technology: General Introduction What is plant tissue culture?

Tissue culture Ti lt is i the th term t usedd for f “the “th process off growing i cells ll artificially in the laboratory” Tissue culture involves both pplant and animal cells Tissue culture produces clones.

in which all product cells have the same genotype (unless affected by mutation during. This comprehensive book on plant tissue culture covers the fundamental as well as applied aspects of this area.

It will be a very useful textbook for students and researchers interested in this field. Intro to Tissue Culture Running time:7min Derek Gilman explores Dark Heart Nursery with Dan Grace and their innovative techniques for cloning cultivars, the latest in tissue breeding, plant genetics, rejuvenating old seeds and what exciting prospects lie ahead in cannabis plant genetics.

Growing plants is obviously important for their many products -food, fiber, medicinal products, aesthetic value and energy.

The significance of plants to our economy leads to efforts to growing fields of biotechnology to provide new methods of plant improvement. Introduction to Plant Cell, Tissue and Organ Culture - Kindle edition by Sunil D. Purohit.

Download it once and read it on your Kindle device, PC, phones or tablets. Use features like bookmarks, note taking and highlighting while reading Introduction to Plant Cell, Tissue and Organ Culture.5/5(1).

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